Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metabolite profiling in blood plasma.

Oliver Fiehn1, Tobias Kind

  • 1Genome Center, University of California-Davis, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heightened innate immunity may trigger chronic inflammation, fatigue and post-exertional malaise in ME/CFS.

npj metabolic health and disease·2025
Same author

Metabolome informs about the chemical exposome and links to brain health.

Environment international·2025
Same author

A standardized nontargeted metabolomics method for cross-laboratory comparison of food profiles.

Food chemistry·2025
Same author

Heightened innate immunity may trigger chronic inflammation, fatigue and post-exertional malaise in ME/CFS.

medRxiv : the preprint server for health sciences·2025
Same author

Author Correction: A universal language for finding mass spectrometry data patterns.

Nature methods·2025
Same author

Heat-induced phosphatidylserine changes drive HSPA1A's plasma membrane localization.

Cell stress & chaperones·2025

Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) offers broad analysis of blood samples for various health conditions. This method quanties primary metabolites and lipids, despite compromises in specific metabolite accuracy.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Metabolomics

Background:

  • Metabolite profiling, established in the 1980s, is used for diagnosing metabolic disorders.
  • Faster MS/MS methods have overshadowed chromatographic techniques, limiting development for other diseases.
  • Profiling blood samples can reveal specific biochemical responses beyond diagnostics.

Purpose of the Study:

  • To exemplify metabolite profiling strategies using gas chromatography-mass spectrometry (GC-MS).
  • To detail experimental steps for blood plasma metabolite analysis.
  • To highlight the broad scope of GC-MS metabolite profiling.

Main Methods:

  • Gas chromatography-mass spectrometry (GC-MS) for metabolite profiling.
  • Detailed experimental procedures including sample withdrawal, storage, protein precipitation, extraction, concentration, and derivatization.

Related Experiment Videos

  • Data acquisition, raw data processing, and result data transformation.

    • Main Results:

    • GC-MS enables relative quantification of diverse compounds in blood plasma.
    • Analysis includes primary metabolites (sugars, amino acids) and lipids (sterols, fatty acids) without fractionation.
    • Broader analytical scope is achieved, accepting compromises in specific metabolite accuracy.

    Conclusions:

    • Metabolite profiling via GC-MS is a versatile strategy for analyzing complex biological samples.
    • The method allows simultaneous analysis of various metabolite classes, offering a comprehensive biochemical overview.
    • Despite limitations in accuracy for individual metabolites, its broad applicability is valuable for health assessments.