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Digestibility of resistant starch containing preparations using two in vitro models
Caroline Fässler1, Eva Arrigoni, Koen Venema
1Institute of Food Science and Nutrition, ETH Zurich, LFO D28 Schmelzbergstr. 9, 8092, Zürich, Switzerland.
European Journal of Nutrition
|October 13, 2006
Summary
Two in vitro digestion models were compared for their ability to predict resistant starch (RS) reaching the colon. The dynamic model showed consistent results for retrograded long chain tapioca maltodextrins (RTmd), unlike high amylose maize (HAM).
Area of Science:
- Food Science
- Nutritional Science
- Biochemistry
Background:
- Resistant starch (RS) offers health benefits in the human colon.
- Accurate prediction of starch reaching the large intestine is crucial for understanding RS effects.
- Characterizing RS structure and quantity is key to assessing its physiological impact.
Purpose of the Study:
- To compare the efficacy of two in vitro digestion models in simulating human colonic starch digestion.
- To evaluate the digestibility of high amylose maize (HAM) and retrograded long chain tapioca maltodextrins (RTmd) using batch and dynamic in vitro models.
Main Methods:
- In vitro digestion using batch and dynamic models with HAM (RS type 2) and RTmd (RS type 3).
- Characterization of starch preparations before and after digestion using X-Ray, DSC, and measurement of total starch, RS, and protein content.
Main Results:
- Both models showed RTmd indigestibility (60-61 g/100 g), aligning with in vivo ileostomy patient data (59 g/100 g).
- HAM digestion varied between models (58-66 g/100 g RS recovery), indicating sensitivity to experimental parameters.
- Dynamic digestion resulted in lower analytically measured RS and reduced crystallinity for both preparations.
Conclusions:
- The two in vitro digestion methods exhibited differential effects on starch molecule structure.
- Model choice significantly influenced the apparent digestibility of HAM, but not RTmd.
- The dynamic model provided more consistent predictions for RTmd, correlating well with in vivo findings.
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