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Updated: Jul 19, 2026
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Published on: June 13, 2019
Phyllodes tumor with osteosarcomatous differentiation: a comparative immunohistochemical study between epithelial and
Alfredo Ribeiro-Silva1, Leandra Náira Zambelli Ramalho, Sérgio Zucoloto
1Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirdo Preto, SP, Brazil. arsilva@fmrp.usp.br
Aims And Background:
Phyllodes tumor of the breast with osteosarcomatous differentiation is rare and very little is known about its molecular profile.
Methods And Study Design:
An immunohistochemical panel with 37 primary antibodies including cytokeratins, mesenchymal markers, key regulators of the cell cycle, oncogenes, apoptosis-related proteins, metalloproteinases and their inhibitors was performed on a formalin-fixed paraffin-embedded sample of phyllodes tumor with osteosarcomatous differentiation in a 49-year-old woman.
Results:
Antiapoptotic stimuli (survivin) predominated in sarcomatous cells. Antiproteolytic stimuli (TIMP-1, TIMP-2 and PAI) were preponderant in all cells, a surprising fact in view of the aggressiveness of the neoplasm. The immunoprofile of the osteoblastic and stromal cells was quite similar, except for c-erbB-3, c-myc, cyclin D1 and p21. Both exhibited positive cells for actin, MyoD1 and GFAP.
Conclusions:
Our results suggest that this osteosarcoma may have originated from metaplasia of stromal cells that underwent a malignant change.
