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Updated: Jul 19, 2026
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
[Transgenic mice produced by intracytoplasmic sperm injection]
1Stated Key Laboratories for Agrobiotechnologies, College of Biological Sciences, China Agricultural University, Beijing.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 14, 2006
Summary
Intracytoplasmic sperm injection (ICSI) can produce transgenic mice. Linear DNA integration into the host genome was more efficient than circular DNA during ICSI for creating genetically modified offspring.
Area of Science:
- Reproductive Biology
- Genetics
- Transgenic Animal Production
Context:
- Previous studies successfully produced normal mice via ICSI.
- This study investigates the potential for generating transgenic mice using ICSI.
Purpose:
- To evaluate the efficiency of producing transgenic embryos and offspring using ICSI with sperm and foreign DNA.
- To compare the integration efficiency of linear versus circular pEGFP-N1 plasmid DNA in ICSI-generated transgenic mice.
Summary:
- Sperm exposed to linear or circular pEGFP-N1 DNA were co-injected into oocytes. Transgenic embryos expressing GFP were obtained.
- Offspring were produced from transferred ICSI embryos, with Southern blot confirming gene integration in some pups.
- A higher integration rate of GFP and neomycin genes was observed when using linear DNA compared to circular DNA.
Impact:
- Demonstrates the high efficiency of producing transgenic mice through ICSI.
- Suggests linear DNA is more readily integrated into the host genome than circular DNA during ICSI-mediated transgenesis.
Related Concept Videos
00:53Transgenic Organisms
Overview
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.