Related Experiment Video
Updated: Jul 19, 2026

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
Published on: December 17, 2012
[Expression and localization of estrogen receptor ERalpha and the kinds of ERbeta mRNA on placenta]
Dan Luo1, Shu-yun Liu, Ai-yun Xing
1Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the kinds of ERa, ERbeta mRNA and the localization of ERa, ERbeta on normal human placenta.
Methods:
The expression and localization of ERa,ERbeta on normal human placenta were detected by reverse transcription-ploymerase chain reaction (RT-PCR) and immunhistochemistry (SP-assay).
Results:
There was mRNA expression of ERalpha and ERbeta on human term placenta, and ERbeta was identified with ERbeta5. The ERa immunoreactivity on human term placenta was in villous cytotrophoblastic nucleus, and that of ERbeta was in syncytiotrophoblastic cytoplasm.
Conclusion:
The findings indicate that ERbeta5 is the kind of ERbeta on placenta. It is localizated in the cytoplasm of syncytiotrophoblast and is probably connected with the production of estrogen.
Related Concept Videos
Gonadal and Placental Hormones
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair growth — and...
Regulated mRNA Transport
Cell Specific Gene Expression
Role of ER in the Secretory Pathway
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Regulation of Expression at Multiple Steps
Internal Receptors

