Related Experiment Video
Updated: Jul 19, 2026

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
Published on: March 14, 2011
[Influence of blockade of costimulation on Th1/Th2 cytokines shift in unexplained early recurrent spontaneous]
Wei Fan1, Shang-wei Li, Xi-ru Liu
1Department of Reproduction, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To assess the influence of blockade of costimulation on Th1/Th2 Cytokines shift in unexplained early recurrent spontaneous abortion (URSA).
Methods:
The levels of IL-2, IFN-gamma and IL-4 were determined by ELISA in the supernatant of cultured maternal peripheral blood mononuclear cells (PBMC) from 30 patients of URSA. Cytokine synthesis was induced by activation with antigens from a choriocarcinomer cell line of JEG-3, with CTLA4Ig at concentrations of 10 microg/mL and 1 microg/mL (experiment groups), or with IgG at the same concentrations (control groups).
Results:
The CTLA4Ig groups showed significantly higher levels of Th2 cytokines IL-4 (P < 0.05), lower levels of Th1 cytokines IL-2 (P < 0.05) and the same levels of IFN-gamma (P > 0.05), compared with the control groups of the same concentrations respectively. The same results were found between the experiment groups of different concentration.
Conclusion:
CTLA4Ig could switch T cell responses from Th1 to Th2 in URSA by blockade of CD80/CD86 costimulation.
More Related Videos
06:03Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
Published on: September 20, 2024
09:26In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Published on: October 15, 2013
Related Concept Videos
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Inflammatory Bowel Disease III: Crohn's Disease
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...