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Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids
Published on: June 23, 2023
[Study on the distribution of lactase in the small intestine of SD rats]
Wei He1, Bin Lü, Cheng-yu Huang
1Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu.
Objective:
The article deals with the study on the distribution of lactase and its mRNA in the SD rat intestine.
Methods:
15 of the 3-4 wk old SD rats were selected for this research project. From SD rat duodenum, we scissored the small intestine into 3 segments of each 10 cm length to determine the lactase activities and mRNA levels.
Results:
The lactase activities of all 3 part intestines were declined from the duodenum stage by stage (the upper, middle and lower segment was 0.179, 0.160, or 0.151 U/ml homogenate respectively, P < 0.05), but there were no significant difference of mRNA levels happening among the 3 groups.
Conclusion:
The results suggest that the lactase activity mainly distribute over the upper intestine, although there were lactase along whole intestine.
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