Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 19, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

[The tk gene mutation analysis in WTK1 cell line by multiplex PCR].

Yi-jing Wang1, Pei-qiang Shuai, Li-shi Zhang

  • 1Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|October 14, 2006
PubMed
Summary

Methyl methanesulfonate (MMS), mitomycin (MMC), and sodium azide (NaN3) significantly increase tk gene mutation frequency in WTK1 cells. Analysis revealed hotspots and common exon deletions, indicating mutagenic effects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Community structure of soil diazotrophs in coastal <i>Casuarina equisetifolia</i> varieties and the environmental drivers.

Ying yong sheng tai xue bao = The journal of applied ecology·2026
Same author

Machine learning reveals targets of <i>Gnaphalium hypoleucum</i> DC. flavonoids against rheumatoid arthritis through gut microbiota and anti-inflammation.

Frontiers in immunology·2026
Same author

Case Report: Rare case of secondary gastric body tuberculosis-diagnostic challenges arising from the insidious spread of abdominal tuberculosis.

Frontiers in medicine·2026
Same author

Correction: Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

eLife·2026
Same author

NIR fluorescent substrate-driven discovery of prolyl endopeptidase natural inhibitors and its inhibition of alpha-synuclein aggregation and promotion of autophagy.

Chemico-biological interactions·2025
Same author

A scoring model based on MRI features for predicting early recurrence after surgical resection of hepatocellular carcinoma.

Frontiers in surgery·2025

Area of Science:

  • Molecular biology
  • Genetics
  • Toxicology

Context:

  • The WTK1 cell line is a valuable model for studying gene mutations.
  • Understanding the mechanisms of chemical mutagenesis is crucial for risk assessment.

Purpose:

  • To investigate the mutation spectrum of the tk gene in spontaneous and induced WTK1 mutants.
  • To identify mutation hotspots within the tk gene.

Summary:

  • Exposure to methyl methanesulfonate (MMS), mitomycin (MMC), and sodium azide (NaN3) resulted in a dose-dependent increase in tk gene mutation frequency in WTK1 cells.
  • Multiplex PCR analysis of induced mutants showed a mutation spectrum predominantly involving the loss of exon 4 and exons 5-7.
  • These findings confirm the mutagenic potential of the tested chemicals and highlight specific mutation hotspots in the tk gene.

More Related Videos

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation
07:17

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Published on: August 23, 2024

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

Related Experiment Videos

Last Updated: Jul 19, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation
07:17

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Published on: August 23, 2024

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

Impact:

  • Provides insights into the mutagenic mechanisms of MMS, MMC, and NaN3 at the tk gene locus.
  • Contributes to the understanding of chemical-induced DNA damage and repair pathways.
  • Informs risk assessment strategies for chemical mutagens.