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MIMOX: a web tool for phage display based epitope mapping.

Jian Huang1, Alex Gutteridge, Wataru Honda

  • 1Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. hjian@kuicr.kyoto-u.ac.jp

BMC Bioinformatics
|October 14, 2006
PubMed
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MIMOX is a new free web tool for epitope mapping using phage display. It helps researchers map antibody epitopes from mimotopes and antigen structures, providing accessible and novel results.

Area of Science:

  • Biotechnology
  • Immunoinformatics
  • Structural Biology

Background:

  • Phage display is a versatile technique used in basic and applied research, including drug development and diagnostics.
  • Epitope mapping is a key application of phage display, crucial for understanding antibody-antigen interactions.
  • Existing computational tools for phage display-based epitope mapping often lack convenient online accessibility.

Purpose of the Study:

  • To develop a user-friendly, web-based tool for automated epitope mapping using phage display data.
  • To provide a platform for mapping native antibody epitopes from mimotopes and antigen structures.
  • To enhance accessibility and usability of epitope mapping tools for the research community.

Main Methods:

  • Developed MIMOX, a Perl-based web tool integrating ClustalW for sequence alignment and JalView for visualization.

Related Experiment Videos

  • Implemented algorithms to derive consensus sequences from mimotopes and map them onto antigen structures.
  • Utilized NACCESS for surface accessibility evaluation and Jmol for interactive 3D visualization of potential epitopes.

    • Main Results:

    • MIMOX successfully maps native epitopes using user-supplied mimotopes and antigen structures.
    • The tool reproduces results from existing methods like FINDMAP and 3DEX.
    • MIMOX provides novel and rational epitope mapping insights, validated through case studies.

    Conclusions:

    • MIMOX offers a freely accessible, web-based solution for phage display-based epitope mapping.
    • The tool enhances community access, utilization, and evaluation of epitope mapping resources.
    • MIMOX complements existing epitope mapping programs and facilitates research in antibody-antigen interactions.