[Monoclonal antibodies to antigens of the Rh system]

For the development of monoclonal antibodies, which are useful for typing of Rh-system antigens, human monoclonal antibodies are needed. We have established a method for raising human monoclonal antibodies using EBV-transformation and subsequent stabilisation by fusing with mouse myeloma or heteromyeloma cell-lines. Using this method, we have produced several clones reacting with the D antigen which can be used for typing purposes as well as for research purposes to differentiate epitopes and categories.