Related Experiment Videos
[Preparation of leukocyte-poor thrombocyte concentrates from buffy coat by using a new quadruple bag system]
1Institut für Klinische Immunologie und Transfusionsmedizin der Justus-Liebig-Universität Giessen.
It has repeatedly been shown that platelet concentrates (PC) can be prepared from buffy coat in guadruple Saline(S)-Adenin(A)-Glucose(G)-Mannitol(M) (SAG-M) systems. Their in vivo and in vitro quality was reported to be excellent and their contamination with leucocytes was 10-20 times lower than in PC prepared from platelet rich plasma. We have confirmed these observations (unpublished results). One particular technical problem which we encountered was the appearance of folds in the satellite bag during the second centrifugation step rendering the purity of the PC quite unpredictable. In collaboration with Biotrans, we have developed a modified quadruple bag (733861100) including a special insert (Heraeus Christ, Osterode, FRG) for the centrifugation rotor which has proved advantageous over existing systems and will be briefly described.
It has repeatedly been shown that platelet concentrates (PC) can be prepared from buffy coat in guadruple Saline(S)-Adenin(A)-Glucose(G)-Mannitol(M) (SAG-M) systems. Their in vivo and in vitro quality was reported to be excellent and their contamination with leucocytes was 10-20 times lower than in PC prepared from platelet rich plasma. We have confirmed these observations (unpublished results). One particular technical problem which we encountered was the appearance of folds in the satellite bag during the second centrifugation step rendering the purity of the PC quite unpredictable. In collaboration with Biotrans, we have developed a modified quadruple bag (733861100) including a special insert (Heraeus Christ, Osterode, FRG) for the centrifugation rotor which has proved advantageous over existing systems and will be briefly described.