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Related Experiment Video

Updated: Jul 19, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

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Published on: September 26, 2016

Heart slice NMR.

Tadayuki Uetani¹, Daisuke Yamashita, Juichiro Shimizu

¹Department of Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.

American Journal of Physiology. Heart and Circulatory Physiology

|October 17, 2006

Summary

This study introduces a novel heart slice preparation for phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy. This method allows for accurate assessment of cardiac energy metabolism and intracellular ion concentrations, unaffected by coronary microcirculation.

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Area of Science:

Biochemistry
Cardiology
Physiology

Background:

Traditional cardiac NMR spectroscopy uses whole heart preparations under coronary perfusion.
Perfusate composition changes and drug applications can alter coronary microcirculation, confounding results.
A new method is needed to study cardiac metabolism without affecting microcirculation.

Purpose of the Study:

To report the first phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy study using a heart slice preparation.
To assess cardiac energy status and intracellular ion concentrations in isolated heart slices.
To evaluate the utility of heart slices for drug potency assessment.

Main Methods:

Utilized left ventricular heart slices superfused with extracellular medium.
Performed 31P NMR spectroscopy to measure metabolite concentrations and chemical shifts.
Manipulated extracellular Na+ concentration to investigate Mg2+ homeostasis.

Main Results:

The ratio of phosphocreatine to ATP was approximately 2.1.
Intracellular pH and Mg2+ concentration ([Mg2+]i) were comparable to retrograde perfusion studies.
[Mg2+]i significantly increased upon removal of extracellular Na+, indicating Na+-coupled Mg2+ transport's role.

Conclusions:

Heart slice preparation is a viable method for 31P NMR spectroscopy of the heart.
This technique provides reliable measurements of cardiac energy metabolism and intracellular ion status.
Heart slices can be used to evaluate cardiac drug potency independently of microcirculatory effects.