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Updated: Jul 19, 2026
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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Published on: March 5, 2022
Exploring host-pathogen interactions at the epithelial surface: application of transcriptomics in lung biology
Joost B Vos1, Nicole A Datson, Klaus F Rabe
1Department of Pulmonology, Leiden Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden, The Netherlands.
Summary
Airway epithelial cells and microbes engage in complex communication. High-throughput genomics technologies like Serial Analysis of Gene Expression (SAGE) and DNA microarrays help analyze these host-pathogen interactions.
Area of Science:
- Microbiology
- Genomics
- Immunology
Background:
- The airway epithelium acts as a critical interface for host defense against inhaled microorganisms.
- Understanding the bidirectional communication (cross-talk) between airway epithelial cells and microbes is essential but challenging.
Purpose of the Study:
- To review Serial Analysis of Gene Expression (SAGE) and DNA microarray technologies.
- To illustrate their application in studying host-pathogen interactions, focusing on lung epithelial cells and microorganisms.
Main Methods:
- Overview of high-throughput genomics technologies: DNA microarray analysis and Serial Analysis of Gene Expression (SAGE).
- Application of these technologies to analyze gene expression in host-pathogen interactions.
Main Results:
- Genomics technologies enable large-scale screening of gene expression within single experiments.
- These methods have provided novel insights into host-pathogen interactions.
Conclusions:
- Serial Analysis of Gene Expression (SAGE) and microarray analysis are powerful tools for investigating complex host-pathogen interactions.
- These technologies are crucial for advancing our understanding of lung epithelial cell and microbial communication.
