Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poly(A) on mengovirus RNA.

D H Spector, D Baltimore

    Journal of Virology
    |October 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    The poly(A) tail size on Mengovirus RNA was analyzed in mouse L cells and HeLa cells. Results showed the poly(A) tail length was consistent across both host cells, averaging 50–70 nucleotides.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl.

    The Journal of biological chemistry·1999
    Same author

    Science over politics.

    Science (New York, N.Y.)·1999
    Same author

    Dissecting Fas signaling with an altered-specificity death-domain mutant: requirement of FADD binding for apoptosis but not Jun N-terminal kinase activation.

    Proceedings of the National Academy of Sciences of the United States of America·1999
    Same author

    Specificities of CD40 signaling: involvement of TRAF2 in CD40-induced NF-kappaB activation and intercellular adhesion molecule-1 up-regulation.

    Proceedings of the National Academy of Sciences of the United States of America·1999
    Same author

    The p65 subunit of NF-kappa B is redundant with p50 during B cell proliferative responses, and is required for germline CH transcription and class switching to IgG3.

    Journal of immunology (Baltimore, Md. : 1950)·1999
    Same author

    Essential roles for the Abl and Arg tyrosine kinases in neurulation.

    Neuron·1999
    Same journal

    Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

    Journal of virology·2026
    Same journal

    Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

    Journal of virology·2026
    Same journal

    MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

    Journal of virology·2026
    Same journal

    Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

    Journal of virology·2026
    Same journal

    A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

    Journal of virology·2026
    Same journal

    ATG9A is an essential host factor for parechovirus RNA replication.

    Journal of virology·2026
    See all related articles

    Area of Science:

    • Virology
    • Molecular Biology
    • Biochemistry

    Background:

    • Polyadenylation is a crucial post-transcriptional modification in eukaryotic mRNA.
    • The poly(A) tail plays a significant role in mRNA stability, export, and translation.
    • Mengovirus, a small RNA virus, provides a model system to study viral RNA modifications.

    Purpose of the Study:

    • To determine the size and characteristics of the poly(A) tail on Mengovirus RNA.
    • To investigate whether host cell type influences the poly(A) tail of Mengovirus RNA.

    Main Methods:

    • Mengovirus RNA was isolated from infected mouse L cells and HeLa cells.
    • Poly(U) filter binding assays were used to detect poly(A) sequences.
    • RNase digestion followed by polyacrylamide gel electrophoresis was employed to analyze poly(A) tail size.

    Related Experiment Videos

    Main Results:

    • Mengovirus RNA from both mouse L cells and HeLa cells demonstrated the presence of poly(A) sequences.
    • RNase-resistant poly(A) stretches were successfully isolated and analyzed.
    • The size of the poly(A) on Mengovirus RNA was consistently found to be between 50 and 70 nucleotides, irrespective of the host cell.

    Conclusions:

    • The host cell type does not significantly affect the size of the poly(A) tail on Mengovirus RNA.
    • The observed poly(A) tail size suggests a conserved mechanism or constraint in Mengovirus RNA processing.
    • These findings contribute to understanding viral RNA modification and its host-cell interactions.