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Related Experiment Videos

Sulfated components of enveloped viruses.

A Pinter, R W Compans

    Journal of Virology
    |October 1, 1975
    PubMed
    Summary

    Viral glycoproteins incorporate sulfate, while non-glycosylated proteins do not. This study investigates sulfate labeling in enveloped viruses, revealing cell-type dependent incorporation in some viral proteins.

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    Area of Science:

    • Virology
    • Glycobiology
    • Biochemistry

    Background:

    • Enveloped viruses possess surface glycoproteins crucial for host cell interaction.
    • Post-translational modifications, such as glycosylation, significantly impact viral protein function.
    • Sulfate incorporation into viral components has been observed but not fully characterized.

    Purpose of the Study:

    • To investigate the incorporation of sulfate into viral glycoproteins.
    • To determine if sulfate labeling is specific to glycosylated viral proteins.
    • To identify the cell types and conditions influencing sulfate incorporation.

    Main Methods:

    • Radiolabeling of enveloped viruses with 35SO4(-2) and [3H]glucosamine.
    • Analysis of viral proteins using gel electrophoresis.
    • Enzymatic treatment (hyaluronidase) to characterize non-proteinaceous components.

    Main Results:

    • Sulfation was detected in glycoproteins of SV5, Sendai, Sindbis, Rauscher leukemia virus, and vesicular stomatitis virus.
    • Non-glycosylated viral proteins did not incorporate sulfate.
    • Sulfate incorporation into vesicular stomatitis virus G protein was cell-type dependent.
    • A cell-derived sulfated mucopolysaccharide was identified and removed by hyaluronidase treatment.

    Conclusions:

    • Viral glycoproteins are targets for sulfate incorporation.
    • Sulfate labeling serves as a marker for viral glycoproteins.
    • Cellular environment influences the extent of viral protein sulfation.

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