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Vesiculation of platelets during in vitro aging.
A P Bode1, S M Orton, M J Frye
1Department of Clinical Pathology and Diagnostic Medicine, East Carolina University School of Medicine, Greenville, NC 27858.
Blood
|February 15, 1991
Summary
Storage of platelet concentrates releases membranous microparticles (MP). Inhibitors and reduced container surface area significantly decrease MP formation and associated procoagulant activity, improving platelet concentrate quality.
Area of Science:
- Hematology
- Biotechnology
- Materials Science
Background:
- Membranous microparticles (MP) are released into plasma from stored platelet concentrates (PC).
- MP formation is influenced by storage conditions and platelet activation.
Purpose of the Study:
- To analyze MP populations in stored PC.
- To investigate methods for reducing MP generation during PC storage.
- To characterize the nature and origin of MP in PC.
Main Methods:
- Flow cytometry was used to quantify MP populations based on size.
- Platelet activation inhibitors (prostaglandin E-1, theophylline, aprotinin) and reduced container surface area were tested.
- Platelet factor 3 (PF3) activity and lactic dehydrogenase release were measured.
- Surface antigen analysis identified MP origins.
Main Results:
- Inhibitors and reduced container surface area reduced MP by ~40%.
- Inhibited concentrates showed significantly lower PF3 activity (84%) and lactic dehydrogenase release (61%).
- A strong correlation (rs = .748) was found between PF3 levels and larger MP concentration.
- Most MP were platelet-derived, expressing glycoprotein (GP) IIbIIIa and/or GPIb.
Conclusions:
- Procoagulant MP are released from activated platelets during PC storage.
- Platelet activation is exacerbated by interaction with the storage container.
- Storage conditions can be modified to reduce MP formation and improve PC quality.