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An ectophosphatase activity in Cryptococcus neoformans.

Itallo Collopy-Junior1, Fabiano Ferreira Esteves, Leonardo Nimrichter

  • 1Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, CCS, Bloco H, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, Brazil.

FEMS Yeast Research
|October 18, 2006
PubMed
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Cryptococcus neoformans expresses active surface acid phosphatases that are inhibited by specific compounds. Irreversible inhibition of these ectoenzymes reduces fungal adherence to host cells, suggesting a role in cryptococcosis pathogenesis.

Area of Science:

  • Mycology
  • Biochemistry
  • Pathogenesis

Background:

  • Fungal pathogens express biologically active ectoenzymes.
  • Surface phosphatases on Cryptococcus neoformans (etiologic agent of cryptococcosis) were investigated.

Purpose of the Study:

  • To evaluate the expression and activity of ectophosphatases on C. neoformans.
  • To determine the role of these ectoenzymes in fungal pathogenesis.

Main Methods:

  • Assessed ectophosphatase activity across different C. neoformans isolates.
  • Tested enzyme inhibition using ammonium molybdate, fluoride, orthovanadate, Zn2+, and inorganic phosphate.
  • Evaluated fungal adherence to animal epithelial cells after irreversible enzyme inhibition.

Main Results:

Related Experiment Videos

  • C. neoformans isolates exhibit ectophosphatase activity, independent of capsule size or serotype.
  • The enzyme is an acid phosphatase, inhibited by molybdate, fluoride, and vanadate; only vanadate inhibition is irreversible.
  • Ectophosphatase activity was highest with phosphothreonine as a substrate.
  • Irreversible inhibition of ectophosphatases significantly reduced fungal adherence to epithelial cells.

Conclusions:

  • Cryptococcus neoformans possesses an acid ectophosphatase that plays a role in pathogenesis.
  • Ectoenzyme expression contributes to the adherence of C. neoformans to host epithelial cells, a key step in cryptococcosis development.