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Updated: Jul 19, 2026

Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion
Published on: July 3, 2026
Sublethal concentrations of the aminoglycoside amikacin interfere with cell division without affecting chromosome
Christophe Possoz1, Jason Newmark, Nohemy Sorto
1College of Natural Science and Mathematics, P.O. Box 6850, 800 N. State College Boulevard, Fullerton, CA 92831-3599, USA.
Abstract:
Aminoglycosides bind to the 16S rRNA at the tRNA acceptor site (A site) and disturb protein synthesis by inducing codon misreading. We investigated Escherichia coli cell elongation and division, as well as the dynamics of chromosome replication and segregation, in the presence of sublethal concentrations of amikacin (AMK). The fates of the chromosome ori and ter loci were monitored by visualization by using derivatives of LacI and TetR fused to fluorescent proteins in E. coli strains that carry operator arrays at the appropriate locations. The results showed that cultures containing sublethal concentrations of AMK contained abnormally elongated cells. The chromosomes in these cells were properly located, suggesting that the dynamics of replication and segregation were normal. FtsZ, an essential protein in the process of cell division, was studied by using an ectopic FtsZ-cyan fluorescent protein fusion. Consistent with a defect in cell division, we revealed that the Z ring failed to properly assemble in these elongated cells.
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