Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the therapeutic potential of psychedelics: Fear extinction mechanisms and amygdala modulation.

Psychedelics·2026
Same author

CB1 receptor signaling at the cingulate-striatal circuit is anxiogenic.

Nature communications·2026
Same author

Unprecedented Burning in Tropical Peatlands During the 20th Century Compared to the Previous Two Millennia.

Global change biology·2026
Same author

Epac2-mediated synaptic insertion of Ca<sup>2+</sup>-permeable AMPARs in the nucleus accumbens contributes to incubation of cocaine craving.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2024
Same author

Multimodal machine learning models identify chemotherapy drugs with prospective clinical efficacy in dogs with relapsed B-cell lymphoma.

Frontiers in oncology·2024
Same author

A Pilot Feasibility Trial of an Upper Extremity Assistive System.

Archives of rehabilitation research and clinical translation·2024

Related Experiment Video

Updated: Jul 19, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

UV irradiation induces a postreplication DNA damage checkpoint.

A John Callegari1, Thomas J Kelly

  • 1Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|October 18, 2006
PubMed
Summary

Moderate UV exposure unexpectedly bypasses normal cell cycle checkpoints in fission yeast. Cells delay progression after DNA replication, requiring DNA replication and Chk1 kinase for this response, suggesting a role in postreplication repair.

More Related Videos

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: Jul 19, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic cells possess cell-cycle checkpoints (G(1)/S, intraS, G(2)/M) to respond to DNA damage from UV radiation.
  • UV irradiation can trigger these checkpoints, halting cell-cycle progression to allow for DNA repair.

Purpose of the Study:

  • To investigate the cell-cycle response of fission yeast to moderate UV doses, mimicking sunlight exposure.
  • To determine the role of DNA replication and the Chk1 kinase in the cellular response to UV-induced DNA damage.

Main Methods:

  • Exposing fission yeast cells to moderate UV doses.
  • Analyzing cell-cycle progression and DNA replication.
  • Utilizing checkpoint kinase (Chk1) mutants (Deltachk1) to assess its function.

Main Results:

  • Moderate UV doses did not induce canonical G(1)/S, intraS, or G(2)/M checkpoints.
  • Cells delayed cell-cycle progression for hours after DNA replication completion, irrespective of their initial cell-cycle stage.
  • This delay was dependent on both DNA replication and the Chk1 kinase.
  • UV-irradiated Deltachk1 cells showed chromosome damage and reduced viability only after DNA replication and entry into mitosis.

Conclusions:

  • A novel cell-cycle response to UV occurs after DNA replication, distinct from established checkpoints.
  • This postreplication cell-cycle delay, mediated by Chk1, is crucial for preventing chromosome damage and maintaining viability.
  • The findings suggest a significant physiological role for this response in facilitating postreplication repair of UV-induced DNA lesions.