Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

UV irradiation induces a postreplication DNA damage checkpoint.

A John Callegari1, Thomas J Kelly

  • 1Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|October 18, 2006
PubMed
Summary

Moderate UV exposure unexpectedly bypasses normal cell cycle checkpoints in fission yeast. Cells delay progression after DNA replication, requiring DNA replication and Chk1 kinase for this response, suggesting a role in postreplication repair.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the therapeutic potential of psychedelics: Fear extinction mechanisms and amygdala modulation.

Psychedelics·2026
Same author

CB1 receptor signaling at the cingulate-striatal circuit is anxiogenic.

Nature communications·2026
Same author

Unprecedented Burning in Tropical Peatlands During the 20th Century Compared to the Previous Two Millennia.

Global change biology·2026
Same author

Epac2-mediated synaptic insertion of Ca<sup>2+</sup>-permeable AMPARs in the nucleus accumbens contributes to incubation of cocaine craving.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2024
Same author

Multimodal machine learning models identify chemotherapy drugs with prospective clinical efficacy in dogs with relapsed B-cell lymphoma.

Frontiers in oncology·2024
Same author

A Pilot Feasibility Trial of an Upper Extremity Assistive System.

Archives of rehabilitation research and clinical translation·2024

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic cells possess cell-cycle checkpoints (G(1)/S, intraS, G(2)/M) to respond to DNA damage from UV radiation.
  • UV irradiation can trigger these checkpoints, halting cell-cycle progression to allow for DNA repair.

Purpose of the Study:

  • To investigate the cell-cycle response of fission yeast to moderate UV doses, mimicking sunlight exposure.
  • To determine the role of DNA replication and the Chk1 kinase in the cellular response to UV-induced DNA damage.

Main Methods:

  • Exposing fission yeast cells to moderate UV doses.
  • Analyzing cell-cycle progression and DNA replication.
  • Utilizing checkpoint kinase (Chk1) mutants (Deltachk1) to assess its function.

Related Experiment Videos

Main Results:

  • Moderate UV doses did not induce canonical G(1)/S, intraS, or G(2)/M checkpoints.
  • Cells delayed cell-cycle progression for hours after DNA replication completion, irrespective of their initial cell-cycle stage.
  • This delay was dependent on both DNA replication and the Chk1 kinase.
  • UV-irradiated Deltachk1 cells showed chromosome damage and reduced viability only after DNA replication and entry into mitosis.

Conclusions:

  • A novel cell-cycle response to UV occurs after DNA replication, distinct from established checkpoints.
  • This postreplication cell-cycle delay, mediated by Chk1, is crucial for preventing chromosome damage and maintaining viability.
  • The findings suggest a significant physiological role for this response in facilitating postreplication repair of UV-induced DNA lesions.