Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionic currents in crustacean neurosecretory cells.

C G Onetti1, U García, R F Valdiosera

  • 1Departamento de Fisiologia, Biofisica y Neurociencias, CINVESTAV, Mexico, D.F.

Journal of Neurophysiology
|November 1, 1990
PubMed
Summary

Investigating crayfish X-organ neurosecretory cells reveals distinct electrical properties. Calcium currents are crucial for neuronal activity, with tetrodotoxin-sensitive sodium currents absent in axotomized cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diet selection and performance of horses grazing on different heathland types.

Animal : an international journal of animal bioscience·2017
Same author

Comparison of feed intake, digestion and rumen function among domestic ruminant species grazing in upland vegetation communities.

Journal of animal physiology and animal nutrition·2016
Same author

Productive responses of breeding Cashmere goats and their kids to different stocking rates on improved upland pastures.

Journal of animal science·2016
Same author

Anthelmintic effect of heather in goats experimentally infected with Trichostrongylus colubriformis.

Parasitology research·2013
Same author

Electrophysiological properties of crayfish retinal photoreceptors.

The Journal of experimental biology·2013
Same author

Effect of the consumption of heather on incoming larvae and established population of Teladorsagia circumcincta in experimentally infected Cashmere goats.

Veterinary parasitology·2013

Area of Science:

  • Neuroscience
  • Cellular Electrophysiology
  • Crustacean Biology

Background:

  • Neurosecretory cells in the crayfish X-organ regulate physiological processes.
  • Understanding their electrical properties is key to deciphering neuroendocrine control.

Purpose of the Study:

  • To characterize the electrical activity and membrane properties of crayfish X-organ neurosecretory cell somata.
  • To identify the ionic currents underlying their firing patterns.

Main Methods:

  • Microelectrode recordings and whole-cell voltage-clamp techniques were employed.
  • Intracellular current injection and tetrodotoxin (TTX) application were used to assess neuronal responses.
  • Calcium and sodium ion concentrations were manipulated.

Related Experiment Videos

Main Results:

  • 56% of cells were silent, 35% showed tonic activity, and 9% exhibited bursting activity.
  • Spiking activity was TTX-sensitive and abolished by axotomy, revealing a Cd2+-sensitive depolarization.
  • A prominent TTX-insensitive calcium current was identified as the main inward current, with reduced currents observed at high intracellular calcium levels.

Conclusions:

  • Crayfish X-organ neurosecretory cells display diverse electrical behaviors.
  • Calcium currents play a primary role in their excitability, while TTX-sensitive sodium currents are not essential for spontaneous activity in axotomized cells.