[UV-induced DNA mutation of peach aphid]

By using PCR technique and microsatellite marks, this paper studied the DNA polymorphism of peach aphid (Myzus persicae) under UV-radiation. The fragments of three primers were amplified, and the gene diversity and the rate of loci polymorphisms of their genomic DNA, which could reflect the damage degree of DNA after UV-radiation, were measured. The results revealed that after treated with different radiation intensity (15, 30, 45 W) and duration (2, 4, 6 h) , the UV-induced DNA mutations were genetic and could be delivered to F2 generation. The mutations depended on the interaction of radiation intensity and duration. Variance analysis on the gene diversity and the rate of loci polymorphisms showed that there existed a significant difference between UV-treated and control groups, except the rate of loci polymorphisms under 2 h radiation. The average value of the control was higher than that of 2 h radiation treatment. According to the cluster analysis of the genetic distance, the aphids were divided into three groups, i. e., control group, 2 h (15, 30 W) treatment group, and the other, which was consistent with the result of variance analysis.