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Updated: Jul 19, 2026
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
Immunolocalization of E-cadherin and alphaE-catenin in rat parotid acinar cell under chronic stimulation of
Fabio D'Amico1, Evangelia Skarmoutsou
1Department of Biomedical Sciences, University of Catania, Catania, Italy. f.damico@unict.it
Abstract:
E-cadherin and alphaE-catenin were localized in normal and chronically isoproterenol-treated acinar cells of rat parotid gland by means of immunogold labelling of Lowicryl embedded sections. Immunostaining of both experimental groups with polyclonal antibodies to E-cadherin and alphaE-catenin was mainly restricted to the areas of adherens junctions. Surprisingly, in isoproterenol-treated cell alphaE-catenin was also found on the secretory granules periphery and appeared to encircle a secretory vesicle. In isoproterenol-induced cell hyperproliferation, the maintened presence of adherens junctions components, such as E-cadherin and alphaE-catenin molecules, should be an essential prerequisite for tissue integrity. Our data suggest the presence of a correlation between the organization of actin and the localization of alphaE-catenin in the chronically isoproterenol-treated acinar cell of rat parotid gland.
