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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Published on: August 6, 2009

Human postmortem tissue: what quality markers matter?

Ana D Stan¹, Subroto Ghose, Xue-Min Gao

¹University of Texas Southwestern Medical Center at Dallas, Department of Psychiatry, 5323 Harry Hines Blvd., NE5.110F, Dallas, TX 75390-9127, USA.

|October 19, 2006

Summary

Postmortem brain tissue quality is crucial for disease research. RNA Integrity Number (RIN) is the best indicator of tissue quality, correlating well with pH, while protein levels remain stable even with RNA degradation.

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Area of Science:

Neuroscience
Biotechnology
Pathology

Background:

Postmortem human brain tissue is vital for studying neurological diseases.
Tissue quality is a critical factor, influenced by pre- and postmortem conditions.
Traditional confounds include agonal factors and long postmortem intervals (PMI).

Purpose of the Study:

To assess tissue quality indicators in postmortem human brain samples.
To correlate RNA quality (RIN) and protein levels with tissue quality parameters.
To identify reliable markers for high-quality postmortem tissue for research.

Main Methods:

Measured tissue quality parameters in over 100 postmortem cases.
Correlated RNA Integrity Number (RIN) with pH and protein levels.
Analyzed protein stability and quality indicators under various conditions (e.g., thawed, surgical tissue).

Main Results:

RNA Integrity Number (RIN) emerged as the most sensitive indicator of tissue quality.
A strong correlation was observed between RIN and pH.
Protein levels remained stable regardless of RNA degradation, showing no correlation with traditional quality factors.
Tissue from Medical Examiner's offices indicated high quality.

Conclusions:

RIN is a reliable metric for assessing postmortem human brain tissue quality.
pH is a useful secondary indicator when correlated with RIN.
Protein stability offers limited insight into overall tissue quality for RNA-based studies.
Standardized collection protocols and source of tissue (e.g., Medical Examiner) are important for ensuring tissue quality.