Related Experiment Video

Updated: Jul 19, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

Inhibition of nuclear factor-kappa B activation reduces glycerol-induced renal injury

Telma de Jesus Soares1, Roberto S Costa, Ana Paula C Balbi

  • 1Department of Physiology, School of Medicine, University of Sao Paulo at Ribeirao Preto, Ribeirao Preto, Sao Paulo, Brazil.

Journal of Nephrology
|October 19, 2006
PubMed
Abstract

Insights

Pyrrolidine dithiocarbamate (PDTC) reduces glycerol-induced kidney injury by inhibiting nuclear factor kappa B (NF-kappa B) activation and decreasing oxidative stress. This antioxidant treatment protects against renal damage and fibrosis in rats.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Toxicology

Background:

  • Glycerol injection causes acute tubular necrosis and interstitial fibrosis.
  • Oxidative stress in glycerol-treated kidneys activates the nuclear factor kappa B (NF-kappa B) pathway.
  • Mitogen-activated protein kinases (MAPKs) are also implicated in renal injury.

Purpose of the Study:

  • To investigate NF-kappa B and MAPK expression in rat renal cortex after glycerol injection.
  • To determine the relationship between NF-kappa B activation and renal structural/functional changes.
  • To evaluate the protective effects of pyrrolidine dithiocarbamate (PDTC), an NF-kappa B inhibitor, against glycerol-induced nephrotoxicity.

Main Methods:

  • Male Wistar rats received intramuscular injections of glycerol, glycerol plus PDTC, or saline.
  • Kidneys were collected at 5 and 30 days post-injection for histological and immunohistochemical analysis.
  • Plasma creatinine, fractional electrolyte excretion, urinary malondialdehyde (MDA), and cortical NF-kappa B expression were measured.

Main Results:

  • Glycerol injection increased plasma creatinine and electrolyte excretion, which were attenuated by PDTC.
  • Increased cortical expression of macrophages and NF-kappa B was observed in glycerol-treated rats.
  • PDTC treatment reduced glycerol-induced histological damage and urinary MDA levels, indicating decreased lipid peroxidation.

Conclusions:

  • PDTC attenuates glycerol-induced renal injury.
  • The protective effect of PDTC is associated with reduced NF-kappa B expression.
  • PDTC mitigates renal damage by decreasing oxidative stress and lipid peroxidation in the renal cortex.

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...