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Updated: Jul 19, 2026
In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
Published on: October 27, 2020
[Effect of delayed preconditioning induced by Sini decoction on myocardial cell apoptosis and its mitochondrial
Ying Liu1, Wei-kang Wu, Chen Chen
1Department of Pathophysiology of Guangdong Pharmacological College, Guangzhou, China. lylygz@hotmail.com
Objective:
To investigate the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND).
Method:
SD rats were divide into four groups: control, sham, I/R and SND groups. The rats in I/R group, left anterior descending coronary artery (LAD) was occluded for 1h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, cytochrome C and bcl-xl were detected by Western blotting and the activity of caspase-3 was detected by assay kit.
Result:
As compared with I/R group, apoptosis rate of myocardial cell, the release of cytochrome C from mitochondria and the activity of caspase-3 were significantly decreased, and the expression of bcl-xl protein was elevated in SND group.
Conclusion:
The delayed preconditioning induced by Sini decoction decreased myocardial cell apoptosis. The mechanism may be related to the inhibition of mitochondria signal pathway of apoptosis.
