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[Determination of cyclovirobuxine-D in huangyangning tablets by RP-HPLC with gradient elution]
Yong Chen1, Xue-Jun Fan, Qing Gong
1Zhejiang Institute for Drug Control, Hangzhou 310004, China. zhouzhou0311@126.com
Objective:
To establish a new method for the determination of cyclovirobuxine-D in huangyangning tablets.
Method:
Agilent Zorbax Extend C18 (4.6 mm x 250 mm, 5 microm) column with temperature 40 degrees C was used. The mobile phase was 0.3% diethylamine methanol solution and 0.3% diethylamine water solution, with a linear gradient of 0.3% diethylamine methanol solution from 78% to 95% in 9 minutes, and then maintained for 6 minutes. The flow rate was 0.8 mL x min(-1). Evaporative light scattering detector was used.
Result:
The calibration curve was linear at a range of 0.57-11.44 microg for the cyclovirobuxine D (r = 0.9996). The average recovery was 97.2% and RSD was 1.1% (n = 9).
Conclusion:
This method is rapid, simple, and reproducible, and can be used as a quality control method for this preparation.
