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[Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii]
Ying-yong Zhao1, Xiu-ming Cui, Yun Dai
1Wenshan Prefecture Sanqi Science and Technology Research Institute, China.
Objective:
To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.
Method:
The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).
Result:
The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.
Conclusion:
The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.
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