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Updated: Jul 19, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Fingerprint analysis of composite Folii Isatidis injection by HPLC]
Rui Liu1, Zhi-gang Liu, Lei Li
1School of Pharmacy, Shenyang Pharmaceutical University, China.
Objective:
To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.
Method:
The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1). The UV detection was set at 254 nm.
Result:
A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established.
Conclusion:
This method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection.
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