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Updated: Jul 19, 2026
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
[Extraction, isolation and structure identification of polysaccharide in root of Salvia miltiorrhiza]
Hong Wang1, Qiang Wang, Shun-chun Wang
1Department of Pharmacy, Zhejiang College of Traditional Chinese Medicine, Hangzhou. wanghongxj@yahoo.com
Objective:
To study the polysaccharide of Salvia miltiorrhiza.
Method:
The root was extracted by water and purified preliminarily by alcohol precipitation. The obtained crude polysaccharide purified by ion exchange resin DEAE-Sepharose Fast Flow then bleached with H2O2 and dialysed. Two portions of light yellow powder were obtained through lyophilization and named as SMP 1, SMP 0.5. On the basis of 13C-NMR, DEPT, IR spectra, monosaccharide composite analysis and part monosaccharide composite analysis, their structures were identified.
Result:
The molecular weight of SMP 1, SMP 0.5 was estimated to be 1.39 x 10(6), 4.03 x 10(5), respectively, and the structure of SMP 1 was mainly alpha-(1-->6) D-Glc linked a few alpha-(1-->2) D-Glc, SMP 0.5 was mainly a alpha-(1-->6) D-Glucosan.
Conclusion:
SMP 1 and SMP0.5 were neutral homosaccharides that extracted from S. miltiorrhiza for the first time.
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