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HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[HPLC fingerprint of Rhizoma Polygoni Cuspidati]
Yun-tong Ma1, De-guang Wan, Liang-ke Song
1Pharmacy School of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. my6593@vip.163.com
Objective:
To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati (Polygonum cuspidatum).
Method:
The HPLC separation was carried with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min. The similarity of chromatograms was compared by mean of the software from Zhongnan University.
Result:
The constituents of P. cuspidatum were well separated by HPLC, and the similarity was above 0.80.
Conclusion:
The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.
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