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Published on: March 15, 2017
[Determination of saponin I in extracts of Allium macrostemnon]
Hai-feng Chen1, Nai-li Wang, Yi Dai
1Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China.
Objective:
To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.
Method:
The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.
Result:
The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).
Conclusion:
The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.
