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Updated: Jul 19, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
Published on: July 6, 2019
Detecting the effect of very low amounts of penetrants in lipid bilayers using Raman spectroscopy
Abstract:
We show that, by the influence on the gauche content in the acyl chains, the effect of very low concentrations (order 10(-2) %) of penetrants (here 2,4-dichlorophenol) in lipid bilayers (here dipalmitoyl-glycerophosphatidylcholine) can be experimentally seen in the Raman spectra. The observed effects suggest increased order and are in accordance with previous X-ray diffraction and calorimetric data. These findings show great promise for studying in situ the effect of low amounts of various small molecules on cell membranes using Raman spectroscopy.
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