Related Experiment Video
Updated: Jul 19, 2026
06:09
Rapid Isolation of Single Cells from Mouse and Human Teeth
Published on: October 28, 2021
Immunolocalization of CD44s in human teeth.
Rosalia Leonardi1, Carla Loreto, Rosario Caltabiano
1II Dental Unit, University of Catania, Policlinico Universitario, Italy.
Acta Histochemica
|October 20, 2006
Summary
The standard form of CD44s, a cell adhesion molecule, is present in mature human teeth. This finding suggests CD44s role in tooth cell differentiation and tissue interactions.
Area of Science:
- Oral Biology
- Cell Biology
- Biochemistry
Background:
- CD44s (standard form) is an integral membrane glycoprotein crucial for cell adhesion.
- CD44s and its isoforms are implicated in cell-cell and cell-substrate interactions within the periodontium.
- Limited data exist on CD44s presence in human adult dental hard tissues.
Purpose of the Study:
- To investigate the localization of CD44s in mature human dental hard structures.
- To provide data on CD44s presence in odontoblasts, cementoblasts, and periodontal ligament fibroblasts.
Main Methods:
- Utilized six extracted human premolars from children aged 10-12 years.
- Employed immunohistochemistry with a monoclonal mouse anti-CD44s antibody for CD44s localization.
- Tissue sections were incubated overnight at 4°C after antibody application.
Main Results:
- CD44s was detected in odontoblasts, periodontal ligament fibroblasts, and cementoblasts of mature human teeth.
- Evidence confirms CD44s localization in these critical dental cell types.
- The study identified CD44s in cementocytes as well.
Conclusions:
- CD44s is localized in key cells of mature human teeth, including odontoblasts, cementocytes, and periodontal ligament fibroblasts.
- CD44s may play a role in tooth cell differentiation and subsequent cell-matrix interactions.
- Potential involvement in inflammatory cell accumulation in dental connective tissues is suggested.
