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[The preparation of blood samples for the automatic measurement of erythrocyte size]
Abstract:
The following procedure is proposed: fixation for 12 h in low ionic strength solution (4 percent formaldehyde in 50 mM sodium phosphate buffer), drying of the suspension drop on the slide, gallocyanin staining. All red cells were contrast-stained without light central spot. The sizes of different red cell groups on the slide differed by less than 5 percent.