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Related Experiment Videos

Modeling of encapsulated cell systems.

Jeffrey D Gross1, I Constantinidis, A Sambanis

  • 1Georgia Tech-Emory Center for the Engineering of Living Tissues, Atlanta, GA 30332, USA.

Journal of Theoretical Biology
|October 20, 2006
PubMed
Summary
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Mathematical models track tissue construct remodeling, linking dissolved oxygen (DO) to cell viability. This helps understand and monitor engineered tissues, like pancreatic cell substitutes, during development.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Mathematical Biology

Background:

  • Tissue engineered substitutes remodel over time due to cell growth and death.
  • Inert matrices lead to remodeling primarily driven by dissolved oxygen (DO) concentration.
  • Alginate-encapsulated insulin-secreting cells for pancreatic substitution exhibit this remodeling, forming peripheral viable cell rings and inner necrotic zones.

Purpose of the Study:

  • To develop and analyze a mathematical model for encapsulated cell systems.
  • To track viable and dead cell densities and DO concentration within the construct.
  • To correlate intrabead DO with cell distribution and viability.

Main Methods:

  • Development of a mathematical model for spherical encapsulated cell systems.

Related Experiment Videos

  • Simulation of viable/dead cell densities and DO concentration over time and radial position.
  • Comparison of model simulations with experimental histology data.
  • Correlation analysis between average intrabead DO (AIDO) and cell number/distribution.

    • Main Results:

    • The model accurately tracks cell density and DO distribution changes during remodeling.
    • Correlations were established between AIDO and total viable cell number.
    • Correlations were also found between AIDO and radial cell/DO distributions.
    • AIDO measurement using (19)F NMR spectroscopy with perfluorocarbon emulsion enables tracking of construct remodeling.

    Conclusions:

    • Mathematical models are valuable for describing dynamic changes in tissue constructs.
    • Models, when integrated with experimental measurements, provide additional insights into system behavior.
    • The developed model and correlations can be used to monitor tissue construct remodeling in vitro and potentially in vivo.