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Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

A method of combined single-cell electrophysiology and electroporation

Lyle J Graham1, Ricardo Del Abajo, Thomas Gener

  • 1Laboratory of Neurophysics and Physiology, CNRS UMR 8119, UFR Biomédicale de l'Université René Descartes, 45 rue des Saint-Pères, 75006 Paris, France. lyle@biomedicale.univ-paris5.fr

Journal of Neuroscience Methods
|October 20, 2006
PubMed

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Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...