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Triple post-transcriptional control.

D H Bechhofer1

  • 1Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029.

Molecular Microbiology
|September 1, 1990
PubMed
Summary

The ermC gene in Bacillus subtilis provides resistance to MLS antibiotics. Its expression is controlled post-transcriptionally through inducible synthesis of a ribosomal RNA methylase.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The ermC gene confers resistance to macrolide, lincosamide, and streptogramin B (MLS) antibiotics.
  • This resistance is mediated by the ermC gene product, a ribosomal RNA methylase.
  • Gene expression is inducible by subinhibitory concentrations of erythromycin.

Purpose of the Study:

  • To investigate the regulatory mechanisms of ermC gene expression in Bacillus subtilis.
  • To understand how MLS antibiotic resistance is controlled at the post-transcriptional level.

Main Methods:

  • Analysis of ermC gene expression in Bacillus subtilis.
  • Investigating post-transcriptional regulatory events including translational attenuation, translational autoregulation, and mRNA stabilization.

Main Results:

  • ermC gene expression is tightly regulated at the post-transcriptional level.
  • Three distinct mechanisms contribute to the regulation: translational attenuation, translational autoregulation, and messenger RNA stabilization.
  • The ribosomal RNA methylase synthesis is induced by erythromycin.

Conclusions:

  • Bacillus subtilis employs sophisticated post-transcriptional control over ermC gene expression.
  • This regulation ensures efficient MLS antibiotic resistance.
  • Understanding these mechanisms can inform strategies against antibiotic resistance.

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