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Related Experiment Videos

The expression of Flrt3 during chick limb development.

Terence G Smith1, Cheryll Tickle

  • 1Division of Cell and Developmental Biology, University of Dundee, Dundee, UK. tel_smith@yahoo.co.uk

The International Journal of Developmental Biology
|October 20, 2006
PubMed
Summary
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Fibronectin-Leucine-Rich Transmembrane protein 3 (FLRT3) shows dynamic expression during chick embryonic limb development. This protein is crucial for apical ridge formation and limb bud outgrowth, potentially regulating cell adhesion.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The Fibronectin-Leucine-Rich Transmembrane protein 3 (FLRT3) gene encodes a transmembrane protein involved in cell signaling and adhesion.
  • FLRT3 is regulated by fibroblast growth factors (FGFs) and has known roles in neurite outgrowth and homotypic cell adhesion.

Purpose of the Study:

  • To investigate the expression pattern of Flrt3 during chick embryonic limb development.
  • To understand the potential role of FLRT3 in limb bud outgrowth and apical ridge formation.

Main Methods:

  • Whole-mount in situ hybridization was employed to visualize Flrt3 gene expression.
  • Expression patterns were analyzed during key stages of chick embryonic limb development.

Main Results:

Related Experiment Videos

  • Flrt3 exhibited dynamic expression during apical ridge formation and limb bud outgrowth.
  • Expression was initially observed in the apical ectodermal ridge and mesenchyme, later forming distinct dorsal and ventral stripes.
  • Abundant expression was detected in the developing hindlimb and limb girdle regions at later stages.

Conclusions:

  • FLRT3 plays a significant role in chick embryonic limb development, particularly during apical ridge formation and outgrowth.
  • The protein may be critical for regulating cellular adhesion between the apical ectodermal ridge and mesenchyme.
  • FLRT3 expression patterns suggest a role in establishing the dorso-ventral positioning of the apical ridge.