Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme annotation with chemical tools.

Ariel G Herman1, Brent R Stockwell

  • 1Department of Biological Sciences, Columbia University, 614 Fairchild Center, 1212 Amsterdam Avenue, MC 2406, New York, New York 10027, USA.

Chemistry & Biology
|October 21, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging roles of ferroptosis in modulating the immune landscape of glial tumours.

Nature cell biology·2026
Same author

MiR-940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells.

Blood. Red cells & iron·2026
Same author

Dissecting Polypharmacology in Phenotypic Screening to Resolve Ferroptotic and Necrotic Cell-Death Mechanisms.

ACS medicinal chemistry letters·2026
Same author

When Pathways Converge: Iron, Lipid Peroxidation, and α-Synuclein in Ferroptosis-Driven Dopaminergic Neurodegeneration.

Journal of neurochemistry·2026
Same author

Ferroptosis as an approach to leverage cancer metabolism.

Trends in cell biology·2026
Same journal

The Hedgehog Pathway Effector Smoothened Exhibits Signaling Competency in the Absence of Ciliary Accumulation.

Chemistry & biology·2017
Same journal

DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.

Chemistry & biology·2015
Same journal

Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis.

Chemistry & biology·2015
Same journal

Synthetic Peptides as cGMP-Independent Activators of cGMP-Dependent Protein Kinase Iα.

Chemistry & biology·2015
Same journal

Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery.

Chemistry & biology·2015
Same journal

Vitamin C as Cancer Destroyer, Investigating Sulfhydration, and the Variability in CFTR Interactome.

Chemistry & biology·2015
See all related articles

Researchers characterized a novel enzyme in cancer cells using cell-based systems. This approach avoids lengthy purification, offering a more direct method for understanding enzyme activity in biological contexts.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Characterizing enzyme activity is crucial for understanding cellular processes and disease mechanisms.
  • Traditional in vitro biochemical assays often require extensive protein purification, which can alter enzyme function.
  • Cell-based systems offer a more physiologically relevant environment for studying enzyme activity.

Discussion:

  • Chiang et al. employed small molecules, RNA interference (RNAi), and metabolomics to investigate a novel enzyme.
  • This multi-pronged approach allowed for comprehensive characterization within the cellular context.
  • The study focused on an enzyme found to be upregulated in cancer cells, suggesting a potential role in tumorigenesis.

Key Insights:

  • A novel enzyme's activity was successfully defined using cell-based methodologies.

Related Experiment Videos

  • The use of RNA interference provided insights into the enzyme's specific function and regulation.
  • Metabolomic analysis revealed the downstream effects of the enzyme's activity on cellular metabolism.

    • Outlook:

    • Further investigation into this enzyme could reveal new therapeutic targets for cancer treatment.
    • The cell-based characterization strategy can serve as a model for studying other enzymes in complex biological systems.
    • Understanding the enzyme's role in cancer metabolism may lead to the development of novel diagnostic biomarkers.