Rapid isolation of rare clones from highly complex DNA libraries by PCR analysis of liquid gel pools
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Updated: Jul 19, 2026
09:19
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
Published on: March 26, 2018
1Harvard College, Cambridge, USA.
Journal of Microbiological Methods
|October 24, 2006
Abstract:
A semiliquid medium was employed in an efficient method to screen highly complex DNA libraries for clones with known sequences. Unbiased clone pools are generated in 2 ml vials and screened by whole-cell PCR, and individual clones are obtained by few additional rounds of dilution and PCR screening. To demonstrate the utility of this approach, the single positive clone present in a 400,000 member metagenomic fosmid library was isolated.
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