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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Carbon Skeletons01:12

Carbon Skeletons

Life on Earth is carbon-based, as all macromolecules that make up living organisms contain carbon atoms. All organic compounds have a carbon backbone. Each carbon atom is tetravalent and can bond with four other atoms, making it an extraordinarily flexible component of biological molecules. Because carbon’s valence electrons are stable, it rarely becomes an ion. As the carbon chain increases in length, structural modifications such as ring structures, double bonds, and branching side chains...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jul 19, 2026

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

SPIRE: the SPIDER reconstruction engine.

William T Baxter1, ArDean Leith, Joachim Frank

  • 1Wadsworth Center, Empire State Plaza, Albany, NY 12201-0509, USA.

Journal of Structural Biology
|October 24, 2006
PubMed
Summary

SPIRE is a new Python program that modernizes user interaction with the SPIDER image processing system for electron microscopy. It offers a graphical user interface and database connectivity for streamlined reconstruction projects.

Area of Science:

  • Microscopy
  • Image Processing
  • Computational Biology

Background:

  • Electron microscopy requires complex image processing for reconstruction.
  • Existing systems like SPIDER can have user interaction challenges.
  • Modernizing scientific software enhances research efficiency.

Purpose of the Study:

  • To develop a user-friendly interface for the SPIDER image processing system.
  • To streamline electron microscopical reconstruction workflows.
  • To improve data management and parameter handling in reconstruction projects.

Main Methods:

  • Developed SPIRE, a Python program with a graphical user interface (GUI).
  • Integrated SPIRE with the SPIDER system for batch file execution.
  • Implemented XML-tagged configuration files for project flexibility.

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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

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Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

  • Enabled database connectivity for parameter download and result upload.

    • Main Results:

    • SPIRE provides an intuitive GUI for SPIDER command execution.
    • Users can easily monitor project status and generated data.
    • Configuration files allow customization for different reconstruction projects.
    • Seamless integration with laboratory databases for parameter and result management.

    Conclusions:

    • SPIRE successfully modernizes user interaction with SPIDER.
    • The software enhances the efficiency and accessibility of electron microscopy reconstruction.
    • SPIRE's flexible design supports diverse research needs in structural biology.