Related Experiment Video
Updated: Jul 19, 2026
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
Published on: March 12, 2022
Comparison of retinal vessel measurements in digital vs film images
Anne Mosher1, Barbara E K Klein, Ronald Klein
1Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School Madison, Madison, Wisconsin 53726, USA. mosher@epi.ophth.wisc.edu
Purpose:
To compare central retinal artery equivalents (CRAE) and central retinal vein equivalents (CRVE) from vessels measured from images captured with a digital camera and digitized images taken with a film camera.
Design:
Comparison of techniques.
Setting:
Clinic.
Patients:
A total of 75 eyes of 48 adults.
Observation Procedures:
Gradings of retinal vessel diameters from images produced by using two photographic techniques: directly from a 45 degrees digital camera and digitized by scanning from a 30 degrees film-based camera.
Main Outcome Measure:
Differences in CRAE and CRVE from two types of images.
Results:
CRAE and CRVE were highly comparable between the two techniques with correlation coefficients of 0.88 and 0.87, respectively.
Conclusion:
Estimates of CRAE and CRVE from measurements taken from digitally captured and digitized film-based images were highly comparable. It is likely that inferences about factors associated with retinal vessel diameters will be unbiased by the method of imaging.
