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Updated: Jul 19, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Essential notes regarding the design of functional siRNAs for efficient mammalian RNAi
Kumiko Ui-Tei1, Yuki Naito, Kaoru Saigo
1Department of Biophysics and Biochemistry, Graduate School of Science and Undergraduate Program for Bioinformatics and Systems Biology, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Abstract:
Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems.
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