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Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

Atomic Absorption Spectroscopy: Overview

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...

Atomic Absorption Spectroscopy: Interference

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

Atomic Absorption Spectroscopy: Instrumentation

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...

Methods for Studying Drug Absorption: In vitro

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

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Related Experiment Video

Updated: Jul 19, 2026

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Published on: July 13, 2016

[Study on cadmium absorption in pumpkin by atomic absorption spectrophotometry].

Zhen-Xia Li¹, Rui-Jun Jing, Wei-Hua Dong

¹Department of Horticulture, Henan College of Science and Technology, Xinxiang 453003, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|October 25, 2006

Summary

Pumpkin cadmium absorption increases with concentration and time, reaching saturation after 8 hours. Absorption is highest in neutral conditions (pH 7) and lowest in acidic conditions (pH 3).

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The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bis(thiourea) Cadmium Chloride Crystals and the Subsequent CdS Obtention

The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bis(thiourea) Cadmium Chloride Crystals and the Subsequent CdS Obtention

Published on: August 30, 2018

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Last Updated: Jul 19, 2026

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Published on: July 13, 2016

The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bis(thiourea) Cadmium Chloride Crystals and the Subsequent CdS Obtention

The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bis(thiourea) Cadmium Chloride Crystals and the Subsequent CdS Obtention

Published on: August 30, 2018

Area of Science:

Environmental Science
Plant Biology
Analytical Chemistry

Context:

Cadmium is a toxic heavy metal pollutant with significant environmental and health implications.
Understanding plant metal uptake is crucial for phytoremediation and food safety.
Pumpkin (Cucurbita) is a widely consumed crop susceptible to heavy metal contamination.

Purpose:

To investigate the quantitative characteristics of cadmium absorption in pumpkin.
To determine the influence of cadmium concentration, time, and pH on cadmium uptake by pumpkin.
To establish optimal conditions for minimizing or maximizing cadmium accumulation in pumpkin.

Summary:

Cadmium absorption in pumpkin significantly increased with rising cadmium concentrations in the growth medium.
Absorption levels demonstrated a time-dependent pattern, reaching saturation after 8 hours of exposure.
The rate of cadmium uptake peaked at 2 hours before declining, indicating a dynamic absorption process.
Pumpkin exhibited reduced cadmium absorption under acidic or alkaline conditions, with minimum uptake at pH 3 and maximum at neutral pH 7.

Impact:

Provides critical data for assessing cadmium risks in agricultural settings and food chains.
Informs strategies for managing cadmium contamination in crops and potentially for phytoremediation applications.
Highlights the importance of soil pH as a key factor influencing heavy metal bioavailability and plant uptake.