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[Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae]
Dan-yi Li1, Ting-ting Wang, Xiao-hui Chen
1School of Pharmacy, Shenyang Pharmaceutical University, China.
Objective:
To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae.
Methods:
HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined.
Results:
The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004".
Conclusion:
The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.
