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Updated: Jul 19, 2026

Aseptic Laboratory Techniques: Plating Methods
Published on: May 11, 2012
Evaluation of two media and different plating techniques for enumeration of thermotolerant coliforms in river water
1Laboratorio de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina. ltamagnini@com.uncor.edu
Abstract:
Testing for evidence of faecal contamination in river water has been traditionally accomplished by enumeration of thermotolerant coliform bacteria. In this work, deoxycholate lactose and m-Endo Agar LES media were evaluated using different techniques. The colony count methods were pour plate technique on Deoxycholate Agar (DCL pour plate), spread plating on m-Endo Agar LES (ELS spread), and membrane filtration on m-Endo Agar LES (ELF filtration). Typical thermotolerant coliform colonies were analysed by conventional biochemical tests. The three matched pairs showed statistically significant differences (P < 0.05) by the Wilcoxon signed rank test. One hundred and twenty three isolates obtained on m-Endo Agar LES (65%) were confirmed as E. coli. Likewise, one hundred and twenty isolates obtained on Deoxycholate Agar (71%) were confirmed as E. coli. The results of this study showed that the matched DCL-ELF presented the smaller statistically significant difference (P = 0.042) so, DCL could be used as alternative to ELF.
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