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[Differentiation of HL-60 cells induced by realgar nano-particles]
Li-Yun Luo1, Tian-Lan Zhang, Kui Wang
1Peking University School of Pharmaceutical Sciences, Beijing 100083, China.
Objective:
To investigate the proliferation inhibition and the differentiation effects of realgar (As4S4) nano-particles on human acute myeloid leukemia cell line HL-60.
Method:
Cell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen (CD11b and CD14) expression assays.
Result:
HL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0 micromol x L(-1) realgar caused a great increase in NBT reduction ability. The expressions of CD11b and CD14 were augmented in cells treated with 0.50 micromol x L(-1) realgar for 48 h, and cell cycles were arrested in G1 phase.
Conclusion:
Low dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.
