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Published on: July 21, 2023
Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface
Shiping Fang1, Hye Jin Lee, Alastair W Wark
1Department of Chemistry, University of California-Irvine, Irvine, CA 92697, USA.
Abstract:
Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
