Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface and interface control on photochemically initiated immobilization.

Li Liu1, Mark H Engelhard, Mingdi Yan

  • 1Department of Chemistry, Portland State University, P O Box 751, Portland, OR 97207-0751, USA.

Journal of the American Chemical Society
|October 26, 2006
PubMed
Summary

Controlling surface azido group density with photoactive cross-linkers is key for polymer immobilization. Adjusting this density impacts film thickness and structure, from smooth films to individual polymer molecules.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unc45b forms a cytosolic complex with Hsp90 and targets the unfolded myosin motor domain.

PloS one·2008
Same author

Evaluation of red CdTe and near infrared CdHgTe quantum dots by fluorescent imaging.

Journal of nanoscience and nanotechnology·2008
Same author

Exploring feasibility of multicolored CdTe quantum dots for in vitro and in vivo fluorescent imaging.

Journal of nanoscience and nanotechnology·2008
Same author

[Effects of Dahuang Zhechong Pill on expressions of tissue inhibitor of metalloproteinase-1 and plasminogen activator inhibitor-1 in rats with glomerulosclerosis].

Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·2008
Same author

[Construction and expression of a fusion protein containing extracellular domain of human Delta-like1 and Fc fragment of human IgG1 in the Pichia pastoris].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2008
Same author

[Preparation and characterization of the monoclonal antibodies to PTD-bcr/abl].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2008

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Surface Chemistry

Background:

  • Surface and interface properties significantly influence photochemical immobilization efficiency.
  • Controlling the density of photoactive groups on a surface is crucial for directed polymer immobilization.

Purpose of the Study:

  • To investigate how surface azido group density affects polymer immobilization using a silane-functionalized perfluorophenyl azide (PFPA-silane) photo-crosslinker.
  • To understand the role of surface topography and additive competition in photochemical immobilization.

Main Methods:

  • Utilized PFPA-silane as a photoactive cross-linker to immobilize polymers.
  • Varied the density of surface azido groups by diluting the photolinker and adding nonphotoactive silanes (PTMS, ODTMS).

Related Experiment Videos

  • Analyzed the impact of surface topography and additive shielding on film thickness and structure.

    • Main Results:

    • Decreased PFPA-silane concentration led to lower azido group density and reduced immobilized film thickness.
    • Nonphotoactive silanes competed with PFPA-silane, accelerating the decrease in film thickness.
    • Longer chain additives (ODTMS) more effectively shielded azido groups, causing a faster reduction in film thickness compared to shorter chain additives (PTMS).
    • Reduced azido group density transitioned immobilized polymer structures from smooth films to patched structures and individual molecules.

    Conclusions:

    • Surface azido group density is a critical parameter for controlling polymer film thickness and morphology during photochemical immobilization.
    • Additive silanes can modulate immobilization by competing for surface sites or altering topography.
    • The study demonstrates a method to tune polymer immobilization from continuous films to discrete molecular assemblies.